Application
Western blot
Sample
Mouse Tissue lysate - whole (spleen)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
80 µg
Specification
spleen
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% milk in TBS-T
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: anti-rabbit secondary antibody
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 30 second(s)
Bands
Specific: 36 kDa Non-specific: 16, 75, 90 kDa
Additional data
Additional Notes
Lane 1: mouse spleen 20ug
Lane 2: mouse spleen 40ug
Lane 3: mouse spleen 80ug
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Xiuzhe Wang
Verified customer
投稿 Jan 21 2012