Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample
Human Tissue sections (skin)
Specification
skin
Permeabilization
No
Fixative
Acetone
Other product details
Concentration
10 µg/ml
Incubation time
19 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA/10% goat serum/1xPBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 647 Goat Anti-Rabbit IgG (H+L) Antibo
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Dilution
1/250
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 19 2013