Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (OPC culture)
Specification
OPC culture
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Other product details
Dilution
1/1000
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 0.3% TritonX with 0.1x PBS- 10% Donkeys
Secondary antibody
Name
Non-Abcam antibody was used: anti mouse Alexa Fluro 568
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 568
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 568
Dilution
1/1000
Additional data
Additional Notes
ICC/IF image of (ab 22510): Cultured OPC were stained with monoclonal anti-FOXP3. The cells were 4% formaldehyde fixed (5min) and then incubated in 10% normal donkeys serum in 0.1% PBS-0.3% TritonX for 30 minutes to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22510, 1/1000 dilution) four hours at +24°C. The secondary antibody (red) was Alexa Fluor® 568 donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Hoechst was used to stain the cell nuclei (blue) at a concentration of 1.43µM. FOXP3 expressed in the nucleus and perinuclear in OPC.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ruma Raha-Chowdhury
Verified customer
投稿 Feb 13 2012