Application
Western blot
Sample
Human Cell lysate - whole cell (LNCap (prostate cancer cell line))
Loading amount
10 µg
Specification
LNCap (prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing (7% tris-acetate)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBS-tween
Secondary antibody
Name
Non-Abcam antibody was used: Rabbit anti goat
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 95 kDa Non-specific: Many kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 02 2008