Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Ewing's sarcoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, pH6.0, 20min 100C
Permeabilization
No
Specification
Ewing's sarcoma
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
30 minute(s) · Temperature: 25°C · Diluent: Dako diluent, background reducers
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: Leica Refine polymer detection
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
Various retrieval regimens tested (none, proteinase, citrate, EDTA). Citrate gave best signal to background reaction. Full procedure automated on Leica Bond RX
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投稿 Dec 15 2016