Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (embryonic brain)
Specification
embryonic brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0,2% Triton X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: RT°C
Other product details
Dilution
1/250
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS with 2% BSA
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG 488 Invitrogen A21206
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
I rate this antibody as “ok/good”. This antibody gave a positive staining result in coronal midbrain sections of stage 22 chicken embryos. The staining wasn't particularly strong and gave some background haziness, however specific staining was identifiable and discernable from background. Images show brightfield, DAPI in blue and Foxa2 staining in green (Scale bar 200 µm).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Judit Kuechler
Verified customer
投稿 Jan 11 2011