Anti-FMN2 抗体 (ab72052)
Key features and details
- Rabbit polyclonal to FMN2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FMN2 antibody
FMN2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FMN2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide (Human) corresponding to a region between residues 650-700 of human FMN2. (NP_064450.3).
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ポジティブ・コントロール
- human brain lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72052の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | ||
WB |
特記事項 |
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Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Required for asymmetric spindle positioning, asymmetric oocyte division and polar body extrusion during female germ cell meiosis (By similarity). Actin-binding protein that is involved in actin cytoskeleton assembly and reorganization. Acts as an actin nucleation factor and promotes assembly of actin filaments together with SPIRE1 and SPIRE2. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Plays a role in responses to DNA damage, cellular stress and hypoxia by protecting CDKN1A against degradation, and thereby plays a role in stress-induced cell cycle arrest. Protects cells against apoptosis by protecting CDKN1A against degradation. -
組織特異性
Expressed almost exclusively in the developing and mature central nervous system. -
配列類似性
Belongs to the formin homology family. Cappuccino subfamily.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain. -
細胞内局在
Cytoplasm > cytoskeleton. Cytoplasm > perinuclear region. Cytoplasm > cytosol. Cell membrane. Cytoplasmic vesicle membrane. Cytoplasm > cell cortex. Nucleus > nucleolus. Recruited to the membranes via its interaction with SPIRE1. Colocalizes with the actin cytoskeleton. Detected at the cleavage furrow during asymmetric oocyte division and polar body extrusion. - Information by UniProt
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参照データベース
- Entrez Gene: 56776 Human
- Omim: 606373 Human
- SwissProt: Q9NZ56 Human
- Unigene: 24889 Human
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別名
- FMN 2 antibody
- Fmn2 antibody
- FMN2_HUMAN antibody
see all
画像
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All lanes : Anti-FMN2 antibody (ab72052) at 0.25 µg/ml
Lane 1 : human brain lysate
Lane 2 : human brain lysate with immunising peptide
Predicted band size: 180 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 67 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemical analysis of formalin fixed and paraffin embedded Human brain tissue section staining FMN2 using ab72052 at 1/300 dilution. HRP- labeled was used as the secondary antibody and hematoxylin was used as a nuclei counterstain.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab72052 は 6 報の論文で使用されています。
- Sharma VP et al. SUN-MKL1 Crosstalk Regulates Nuclear Deformation and Fast Motility of Breast Carcinoma Cells in Fibrillar ECM Microenvironment. Cells 10:N/A (2021). PubMed: 34205257
- Li Y et al. A novel circFMN2 promotes tumor proliferation in CRC by regulating the miR-1182/hTERT signaling pathways. Clin Sci (Lond) 133:2463-2479 (2019). PubMed: 31738400
- Xie B et al. Poly(ADP-ribose) mediates asymmetric division of mouse oocyte. Cell Res 28:462-475 (2018). PubMed: 29463901
- Yang Z et al. Nuclear Envelope Rupture Is Enhanced by Loss of p53 or Rb. Mol Cancer Res 15:1579-1586 (2017). PubMed: 28811362
- Skau CT et al. FMN2 Makes Perinuclear Actin to Protect Nuclei during Confined Migration and Promote Metastasis. Cell 167:1571-1585.e18 (2016). PubMed: 27839864
- Lynch J et al. Metastasis Suppressor microRNA-335 Targets the Formin Family of Actin Nucleators. PLoS One 8:e78428 (2013). WB ; Human . PubMed: 24223803