Application
Western blot
Sample
Rat Tissue lysate - whole (Eskeletal muscle)
Loading amount
30 µg
Specification
Eskeletal muscle
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Diluent: 0.5%MILKT-PBS0.1%
Secondary antibody
Name
Dilution
1/500
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 90, 55 kDa Non-specific: 65 kDa
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投稿 May 27 2009