Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain Tissue)
Loading amount
40 µg
Specification
Brain Tissue
Gel Running Conditions
Non-reduced Denaturing (10% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/5000
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Milk in TBST
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Dilution
1/5000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~48 kDa
Additional data
Additional Notes
Bands 1-2: Hippocampus tissue
Bands 3-4: Cortex
Bands 5-6: Cerebellum
Bands 3-4: Cortex
Bands 5-6: Cerebellum
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 27 2011