Application
Western blot
Sample
Rat Cell lysate - whole cell (PC12)
Loading amount
30 µg
Specification
PC12
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Concentration
0.1 µg/ml
Incubation time
60 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% Milk, 0.1% Tween-20, PBS
Secondary antibody
Name
Non-Abcam antibody was used: Swine anti rabbit-HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Supersignal West Pico
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: ~25 kDa
Additional data
Additional Notes
Good Clean Antibody
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 20 2007