Application
Western blot
Sample
Hamster Cell lysate - whole cell (CHO)
Loading amount
5 µg
Specification
CHO
Gel Running Conditions
Reduced Denaturing (10-20% tris-glycine gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/2000
Incubation time
2 hour(s) and 30 minute(s) · Temperature: 23°C · Diluent: 1% NFDM-TBST
Secondary antibody
Name
Non-Abcam antibody was used: HRP-conjugated Goat anti-Mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
Chemiluminescence
Exposure
5 second(s)
Bands
Specific: 12 kDa
Positive control
The lysate was a positive control.
Negative control
I did not have a negative control.
Additional data
Additional Notes
I used a nitrocellulose membrane.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 17 2008