Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic fibroblasts)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount
500000 cells
Treatment
pretreated with IFNbeta for 24 hours / 25ng/mL TNFalpha + 50uM zVAD for additional 5 hours
Specification
mouse embryonic fibroblasts
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye 680RD Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680
Detection
Detection method
Near-Infrared Fluorescence Imaging
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 12-13 kDa
Positive control
1st lane: wild-type MEFs pretreated with IFN beta overnight
2nd lane: wild-type MEFs pretreated with IFN beta overnight and then treated with 25ng/mL TNFalpha and 50uM zVAD for additional 5 hours
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 06 2016