Application
ChIP
Detection step
Real-time PCR
Sample
Human cytomegalovirus Cell lysate - nuclear (Human cytomegalovirus infected HF cells)
Specification
Human cytomegalovirus infected HF cells
Negative control
mouse IgG used as control antibody (from the kit (Invitrogen Magnify Chromatin Immunoprecipitation System))
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 5 minute(s) and 0 second(s)
Specification of the cross-linking agent: 37.5% formaldehyde
Duration of cross-linking step: 5 minute(s) and 0 second(s)
Specification of the cross-linking agent: 37.5% formaldehyde
Positive control
Input sample
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Additional data
Additional Notes
1. The experiment was to detect FKBP12-tagged human cytomegalovirus pUL79 protein at viral loci from viral infected cell lysates
2. ChIP signals of FKBP12-pUL79 fusion protein: ADddUL79-FKBP12
ChIP signals of control IgG: ADddUL79-IgG
3. The antibody can be used for ChIP assay to detect FKBP12-conjugated proteins
4. 6ug anti-FKBP12 or control IgG antibodies were added into each chromatin sample (extracted from 2.5X10^5 cells)
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投稿 Mar 25 2015