Application
Western blot
Sample
Rat Purified protein (Protein purified from bacteria)
Loading amount
14 µg
Specification
Protein purified from bacteria
Gel Running Conditions
Non-reduced Denaturing (4-20% Tris-Glycine Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 680 goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/10000
Detection
Detection method
Licor
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 12 kDa Non-specific: 8, 10 kDa
Additional data
Additional Notes
First Lane: Marker: 10kD, 15kD, 20kD from the bottom up. Second Lane: Blank (slight spill over). Third Lane: Recombinant FKBP12 that is tagged, thus it runs at a slightly higher kD.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 19 2008