Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Loading amount
20 µg
Specification
Heart
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/2000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% non-fat dry milk in 1X TBST
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit HRP conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/7500
Detection
Detection method
Chemiluminescence
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 19 kDa Non-specific: 60 kDa
Additional data
Additional Notes
Nitrocellulose membranes used.
Lanes 1 and 2 in image are both cardiac whole tissue lysates.
Lanes 1 and 2 in image are both cardiac whole tissue lysates.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 02 2008