製品の概要

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Ammonium Sulphate Precipitation
  • 特記事項(精製)
    ab75928 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Predicted molecular weight: 17 kDa.
IHC-P 1/10 - 1/50.
ELISA 1/1000.

ターゲット情報

  • 機能
    The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
  • 配列類似性
    Belongs to the heparin-binding growth factors family.
  • 細胞内局在
    Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Acidic fibroblast growth factor antibody
    • aFGF antibody
    • Beta endothelial cell growth factor antibody
    • Beta-endothelial cell growth factor antibody
    • ECGF antibody
    • ECGF beta antibody
    • ECGF-beta antibody
    • ECGFA antibody
    • ECGFB antibody
    • Endothelial Cell Growth Factor alpha antibody
    • Endothelial Cell Growth Factor beta antibody
    • FGF 1 antibody
    • FGF alpha antibody
    • Fgf1 antibody
    • FGF1_HUMAN antibody
    • FGFA antibody
    • Fibroblast Growth Factor 1 Acidic antibody
    • Fibroblast growth factor 1 antibody
    • GLIO703 antibody
    • HBGF 1 antibody
    • HBGF-1 antibody
    • HBGF1 antibody
    • Heparin binding growth factor 1 antibody
    • Heparin binding growth factor 1 precursor antibody
    • Heparin-binding growth factor 1 antibody
    see all

画像

  • All lanes : Anti-FGF1 antibody - N-terminal (ab75928) at 1/50 dilution

    Lane 1 : Non transfected 293T cell lysate
    Lane 2 : 293T cell lysate transiently transfected with the FGF1 gene

    Lysates/proteins at 12.5 µg per lane.


    Predicted band size : 17 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of FGF1 in formalin-fixed, paraffin-embedded human colon carcinoma tissue using ab75928, at 1/10 dilution, followed by a peroxidase-conjugated secondary antibody and DAB staining.

参考文献

ab75928 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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