Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuro2a cells)
Loading amount
20 µg
Specification
neuro2a cells
Treatment
FCHSD1 shRNA
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Mouse IgG (H+L), HRP Conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 100 kDa
Positive control
Fchsd1 overexpression
Negative control
FCHSD1 KD
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 15 2011