Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 2% TritonX100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: RT°C
Other product details
Dilution
1/100
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% Tween 20 in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-rabbit alexa 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/1000
Additional data
Abcam response
Thank you for your valuable feedback. As far as we are aware, this is the first time this antibody has been used in ICC-IF.
As nuclear clearer staniing woud usually be expected for FANCF, we would be pleased to recieve further feedback from other customers who have used this antibody in ICC-IF to help clarify these results. It may be that with further optmization, clearer nuclear staining could be obtained.
As nuclear clearer staniing woud usually be expected for FANCF, we would be pleased to recieve further feedback from other customers who have used this antibody in ICC-IF to help clarify these results. It may be that with further optmization, clearer nuclear staining could be obtained.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 01 2011