Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF)
Loading amount
30 µg
Specification
MEF
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/2000
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST with 5% milk
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Dilution
1/10000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: none kDa Non-specific: 33, 80, 250 kDa
Additional data
Additional Notes
This antibody does not recognize Fancc in Mouse cells. I have also used this same antibody for human cells and it works good. I would give it 4 stars for human cell lysate.
Abcam response
Thank you for your valuable feedback. This antibody has only been tested and characterized for WB application on human samples. It may well be that it does not recognize mouse FANCC.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 15 2007