Application
Western blot
Sample
Human Cell lysate - whole cell (THP1 cells)
Loading amount
126 µg
Specification
THP1 cells
Blocking step
BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 3%
Other product details
Dilution
1/1500
Incubation time
18 hour(s) and 0 minute(s)
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 60 kDa Non-specific: 55, 67, 70 kDa
Additional data
Additional Notes
Blocked overnight at 5C attempting to reduce non-specifics, but several non-specific bands were observed along with FANCC band.
Abcam response
Thank you for your valuable feedback. We can suggest the background observed in this procedure could be reduced by increasing the percentage of BSA blocking agent to 5%, and incubating with the primary antibody at 4oC.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Wei-Ting Lu
Verified customer
投稿 May 29 2007