Anti-FAN1 抗体 (ab68572)
Key features and details
- Mouse polyclonal to FAN1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FAN1 antibody
FAN1 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to FAN1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human FAN1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- Human spleen lysate. MTMR15 transfected 293T cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68572の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa). |
ターゲット情報
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機能
Nuclease required for maintenance of chromosomal stability. Plays a key role in DNA repair of DNA interstrand cross-links (ICL) by being recruited to sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, possibly in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Has both endonuclease activity toward 5'-flaps and 5'-exonuclease activity: may act in concert with the 3'-flap-specific enzymes to unhook the ICL by cleaving the lagging-strand template. -
配列類似性
Belongs to the FAN1 family.
Contains 1 UBZ-type zinc finger.
Contains 1 VRR-NUC domain. -
ドメイン
The UBZ-type zinc finger specifically binds monoubiquitinated FANCD2. -
細胞内局在
Nucleus. Localizes at sites of DNA damage following recruitment by monoubiquitinated FANCD2. - Information by UniProt
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参照データベース
- Entrez Gene: 22909 Human
- Omim: 613534 Human
- SwissProt: Q9Y2M0 Human
- Unigene: 584863 Human
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別名
- 6030441H18Rik antibody
- Coiled coil domain containing protein MTMR15 antibody
- DKFZp451H236 antibody
see all
画像
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Anti-FAN1 antibody (ab68572) at 1/500 dilution + Human spleen lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 114 kDa
Observed band size: 114 kDa -
All lanes : Anti-FAN1 antibody (ab68572) at 1/500 dilution
Lane 1 : MTMR15 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 114 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (2)
ab68572 は 2 報の論文で使用されています。
- Kim JJ et al. PCAF-Mediated Histone Acetylation Promotes Replication Fork Degradation by MRE11 and EXO1 in BRCA-Deficient Cells. Mol Cell 80:327-344.e8 (2020). PubMed: 32966758
- Wang Z et al. Microarray gene profiling analysis of glioblastoma cell line U87 reveals suppression of the FANCD2/Fanconi anemia pathway by the combination of Y15 and temozolomide. Arch Med Sci 15:1035-1046 (2019). PubMed: 31360198