Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse mammary cell)
Loading amount
50 µg
Specification
Mouse mammary cell
Treatment
5ng/ml TGF beta for different time periods (hrs)
Gel Running Conditions
Reduced Denaturing (10.0% gel)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5 %FBS in TTBS
Secondary antibody
Name
Non-Abcam antibody was used: GE antirabbit HRP
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
GE ECL plus
Exposure
1 minute(s) and 30 second(s)
Bands
Specific: 30 kDa Non-specific: 60, 46, 20 kDa
Positive control
0,1,3,6,12 hrs TGF beta treated whole cell lysate
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投稿 Aug 31 2011