Application
Western blot
Sample
Human Cell lysate - whole cell (Prostate)
Loading amount
25 µg
Specification
Prostate
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% gel)
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/15000
Detection
Detection method
ECL
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 30 kDa Non-specific: 46, 58, 75, 80 kDa
Positive control
MCF-7
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Olayinka Oloko
Verified customer
投稿 Apr 01 2011