Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (mouse embryonic fibroblasts)
Total protein in input
5e+006 cells
Treatment
50 ng/mL TNFalpha for 5 hours
Immuno-precipitation step
Other - Protein G Dynabeads
Specification
mouse embryonic fibroblasts
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Pierce™ IP Lysis Buffer
Dilution
1/400
Additional data
Additional Notes
Lane 1: IP by control IgG antibody
Lane 2: IP by RIPK3 antibody (ProSci)
Lane 3: IP by FADD antibody (Abcam ab124812)
The pulldown lysates were analyzed by western blot using anti-RIPK3 & anti-FADD antibodies. The results suggested that anti-FADD (Ab124812) is good for immunoprecipitation. The ability for co-immunoprecipitatino requires further optimization.
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投稿 May 03 2016