Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Adult brain and lung)
Specification
Adult brain and lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/400
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: Dako anti mouse Igs
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
I have obtained particular patterns of immunostaining with this Ab on rat and mouse brain....and also other mouse organs, particularly in mouse liver.
The submitted composite image shows clear positivity in the cytoplasm of respiratory epithelium ( this is obtained with a dilution factor of 1/500 but is better at 1/600) and also what seems to be macrophage cytoplasm ( upper image).
In the mouse brain, ( lower image) there is clear positivity in the hippocampal CA1/2/3 cortical neurones, and also in oligodendrocytes ( not clear in this LP image).
Yes.... there is also positivity in the dendritic fields of the hippocampus.
I am happy to receive Abcam's response to either delete or resubmit, using this montage.
The submitted composite image shows clear positivity in the cytoplasm of respiratory epithelium ( this is obtained with a dilution factor of 1/500 but is better at 1/600) and also what seems to be macrophage cytoplasm ( upper image).
In the mouse brain, ( lower image) there is clear positivity in the hippocampal CA1/2/3 cortical neurones, and also in oligodendrocytes ( not clear in this LP image).
Yes.... there is also positivity in the dendritic fields of the hippocampus.
I am happy to receive Abcam's response to either delete or resubmit, using this montage.
Abcam response
Thank you for your honest and thoughtful feedback. We have not yet tested this antibody in that application. I would recommend increasing the primary antibody incubation time to overnight at 4C, as this will maximize signal to noise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Jan 08 2009