Anti-FAAH1 抗体 [4H8] (ab54615)
Key features and details
- Mouse monoclonal [4H8] to FAAH1
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-FAAH1 antibody [4H8]
FAAH1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [4H8] to FAAH1 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human FAAH1 aa 480-579.
Sequence:DLNAPGRATGAVSYTMLYNCLDFPAGVVPVTTVTAEDEAQMEHYRGYFGD IWDKMLQKGMKKSVGLPVAVQCVALPWQEELCLRFMREVERLMTPEKQSS
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特記事項
This product was changed from ascites to tissue culture supernatant on 17/04/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituents: 8% Sodium chloride, 0.6% Dibasic monohydrogen sodium phosphate, 0.2% Monobasic dihydrogen potassium phosphate, 0.2% Potassium chloride, 91% Water -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
4H8 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab54615の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.
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ICC/IF |
Use at an assay dependent concentration. PubMed: 24223930
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IP |
Use at an assay dependent concentration.
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IHC-P | (3) |
Use at an assay dependent concentration.
See Abreview. |
特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 63 kDa. |
ICC/IF
Use at an assay dependent concentration. PubMed: 24223930 |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. See Abreview. |
ターゲット情報
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機能
Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. -
組織特異性
Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate. -
配列類似性
Belongs to the amidase family. -
細胞内局在
Endomembrane system. Cytoplasm > cytoskeleton. Seems to be attached to intracellular membranes and a portion of the cytoskeletal network. - Information by UniProt
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参照データベース
- Entrez Gene: 2166 Human
- Entrez Gene: 14073 Mouse
- Entrez Gene: 29347 Rat
- Omim: 602935 Human
- SwissProt: O00519 Human
- SwissProt: O08914 Mouse
- SwissProt: P97612 Rat
- Unigene: 720143 Human
see all -
別名
- Anandamide amidohydrolase 1 antibody
- Anandamide amidohydrolase antibody
- FA2H antibody
see all
画像
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FAAH1 was immunoprecipitated using 0.5mg Mouse Liver whole tissue extract, 5µg of Mouse monoclonal to FAAH1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab54615.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 63kDa: FAAH1.This image was generated using the ascites version of the product.
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FAAH antibody (ab54615) at 1ug/lane + A-431 cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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Anti-FAAH1 antibody [4H8] (ab54615) + Rat Brain
Predicted band size: 63 kDaThis image was generated using the ascites version of the product.
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Anti-FAAH1 antibody [4H8] (ab54615) + PC-12
Predicted band size: 63 kDaThis image was generated using the ascites version of the product.
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Anti-FAAH1 antibody [4H8] (ab54615) + NIH/3T3
Predicted band size: 63 kDaThis image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (27)
ab54615 は 27 報の論文で使用されています。
- Xu C et al. Inhibitory Neurotransmission Is Sex-Dependently Affected by Tat Expression in Transgenic Mice and Suppressed by the Fatty Acid Amide Hydrolase Enzyme Inhibitor PF3845 via Cannabinoid Type-1 Receptor Mechanisms. Cells 11:N/A (2022). PubMed: 35269478
- Pang Z & Ye L A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry. STAR Protoc 3:101778 (2022). PubMed: 36313539
- Angyal Á et al. Anandamide Concentration-Dependently Modulates Toll-Like Receptor 3 Agonism or UVB-Induced Inflammatory Response of Human Corneal Epithelial Cells. Int J Mol Sci 22:N/A (2021). PubMed: 34360541
- Soares PN et al. Late effects of early weaning on food preference and the dopaminergic and endocannabinoid systems in male and female rats. J Dev Orig Health Dis N/A:1-11 (2021). PubMed: 33650480
- Shepard RD & Nugent FS Targeting Endocannabinoid Signaling in the Lateral Habenula as an Intervention to Prevent Mental Illnesses Following Early Life Stress: A Perspective. Front Synaptic Neurosci 13:689518 (2021). PubMed: 34122037