Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (xenograft tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 9 access super buffer
Permeabilization
No
Specification
xenograft tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1 percent BSA / TBS
Concentration
10 µg/ml
Secondary antibody
Name
Non-Abcam antibody was used: Rabbit HRP-Polymer
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Worked well with antigen retrieval at pH 9.5 using a decloaking chamber (110oC for 2 min followed by cooling to 80oC)
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投稿 Nov 13 2015