Anti-FBXO38 抗体 (ab87729)
Key features and details
- Rabbit polyclonal to FBXO38
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-FBXO38 antibody
FBXO38 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FBXO38 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to Human FBXO38 aa 850-950.
Database link: Q6PIJ6 -
ポジティブ・コントロール
- HeLa and NIH3T3 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab87729の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 134 kDa.
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IP |
Use at 5-10 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 134 kDa. |
IP
Use at 5-10 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
F box protein 38 probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. It may coactivate KLF7, but does not seem to promote KLF7 ubiquitination. There are three named isoforms. -
細胞内局在
Cytoplasmic and Nuclear -
参照データベース
- Entrez Gene: 462174 Chimpanzee
- Entrez Gene: 533554 Cow
- Entrez Gene: 489199 Dog
- Entrez Gene: 100060728 Horse
- Entrez Gene: 81545 Human
- Entrez Gene: 107035 Mouse
- Entrez Gene: 307390 Rat
- Entrez Gene: 709869 Rhesus monkey
see all -
別名
- F box only protein 38 antibody
- F box protein 38 antibody
- Fbx38 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (right) and human testicular seminoma (right) tissues labelling FBXO38 with ab87729 at 1/1000 (1µg/ml). Detection: DAB.
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All lanes : Anti-FBXO38 antibody (ab87729) at 0.1 µg/ml
Lane 1 : Whole cell
lysate from HeLa cells at 50 µg
Lane 2 : Whole cell
lysate from HeLa cells at 15 µg
Lane 3 : Whole cell
lysate from HeLa cells at 5 µg
Lane 4 : Whole cell
lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 134 kDa
Observed band size: 134 kDa
Additional bands at: 107 kDa (possible isoform)
Exposure time: 3 minutes -
Detection of FBXO38 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab87729 at 10 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab87729 は論文での使用が確認できていません。