Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain (choroid plexus))
Permeabilization
Yes - Triton X-100
Specification
Brain (choroid plexus)
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: 1% goat serum 0.5% Triton X in PBS
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG secondary antibody Alexa Fluo
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Additional data
Abcam response
Thank you for participating in our AbTrial program and your valuable feedback. We are pleased that this antibody worked well for you for IHC-Fr in mouse, a species and application that has not been tested before. We welcome any further feedback and AbReviews from other researchers who have used this antibody in rat which will help us assess how well the antibody works in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 09 2018