Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Culex pipiens Tissue lysate - whole (whole fly homogenates)
Specification
whole fly homogenates
Treatment
30 minutes after feeding with sheep blood
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000
Detection
Detection method
ECL+
Bands
Specific: 50 kDa
Exposure
10 second(s)
Negative control
None
Positive control
30 minutes after blood feeding mosquitoes with sheep blood
Additional data
Abcam response
new species : mosquito: Culex pipiens
ABTRIAL-TBJSD
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Jiawen Xu
Verified customer
投稿 Aug 20 2013