Anti-ErbB3 / HER3 (phospho Y1289) 抗体 [EPR2325] (ab76469)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2325] to ErbB3 / HER3 (phospho Y1289)
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325]
ErbB3 / HER3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2325] to ErbB3 / HER3 (phospho Y1289) -
由来種
Rabbit -
特異性
This antibody only detects ErbB 3 phosphorylated on Tyrosine 1289.
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アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- SKBR3 cell lysate treated with Neuregulin. MCF7 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2325 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76469の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2500 - 1/5000. Detects a band of approximately 185 kDa (predicted molecular weight: 148 kDa).
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ICC/IF |
1/100.
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特記事項 |
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WB
1/2500 - 1/5000. Detects a band of approximately 185 kDa (predicted molecular weight: 148 kDa). |
ICC/IF
1/100. |
ターゲット情報
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機能
Binds and is activated by neuregulins and NTAK. -
組織特異性
Epithelial tissues and brain. -
関連疾患
Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
発生段階
Overexpressed in a subset of human mammary tumors. -
ドメイン
The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins. -
翻訳後修飾
Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2065 Human
- Omim: 190151 Human
- SwissProt: P21860 Human
- Unigene: 118681 Human
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別名
- c erbB 3 antibody
- c erbB3 antibody
- Erb b2 receptor tyrosine kinase 3 antibody
see all
画像
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Immunofluorescent staining of MCF7 cells using ab76469 at 1/100 dilution.
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All lanes : Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469) at 1/5000 dilution
Lane 1 : SKBR3 cell lysate untreated
Lane 2 : SKBR3 cell lysate treated with Neuregulin
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 148 kDa
Observed band size: 185 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab76469 は 5 報の論文で使用されています。
- Pereira PMR et al. HER2-Targeted PET Imaging and Therapy of Hyaluronan-Masked HER2-Overexpressing Breast Cancer. Mol Pharm 17:327-337 (2020). PubMed: 31804840
- Jacobi N et al. Organotypic three-dimensional cancer cell cultures mirror drug responsesin vivo: lessons learned from the inhibition of EGFR signaling. Oncotarget 8:107423-107440 (2017). PubMed: 29296175
- Humtsoe JO et al. ErbB3 upregulation by the HNSCC 3D microenvironment modulates cell survival and growth. Oncogene 35:1554-64 (2016). PubMed: 26073080
- Sollome JJ et al. HER2/HER3 regulates extracellular acidification and cell migration through MTK1 (MEKK4). Cell Signal 26:70-82 (2013). Human . PubMed: 24036211
- Munk M et al. Hypoxia changes the expression of the epidermal growth factor (EGF) system in human hearts and cultured cardiomyocytes. PLoS One 7:e40243 (2012). WB ; Mouse . PubMed: 22792252