Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast carcinoma and Breast cell lines)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6 and EDTA pH8.5
Permeabilization
No
Specification
Breast carcinoma and Breast cell lines
Blocking step
(agent) for 10 minute(s) · Concentration: 50% · Temperature: 24°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: Biocare Da vinci
Dilution
1/2500
Secondary antibody
Name
Non-Abcam antibody was used: MACH1 DAB Anti-Mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Robust antibody, staining mostly membrane and cytoplasm. Able to produce reasonable staining even at high dilutions > 1:5000.
Note that in HER2- cell lines or tumours, there will still be speckle staining in cytoplasm.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Malcolm Lim
Verified customer
投稿 Jun 27 2017