Anti-ErbB2 / HER2 抗体 [3B5] (ab16901)
Key features and details
- Mouse monoclonal [3B5] to ErbB2 / HER2
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-ErbB2 / HER2 antibody [3B5]
ErbB2 / HER2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3B5] to ErbB2 / HER2 -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WB, Flow Cytmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Dog, Monkey -
免疫原
Synthetic peptide corresponding to Human ErbB2/ HER2 aa 1242-1255 (C terminal).
Sequence:TAENPEYLGLDVPV
Database link: P04626 -
エピトープ
Within amino acids 1242 - 1255 of human c-neu (TAENPEYLGLDVPV) -
ポジティブ・コントロール
- SK-BR-3 cells or breast carcinoma tissue
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特記事項
For paraffin sections, we recommend pretreating with a pressure cooker, but trypsin and heat will also work well. Including 0.05% saponin during staining of paraffin sections may help reduce background. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribriform growth type were stained by this antibody.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
3B5 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Affibody® Molecule
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16901の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (3) |
Use at an assay dependent concentration.
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WB | (5) |
Use a concentration of 2.5 µg/ml. Detects a band of approximately 190 kDa (predicted molecular weight: 138 kDa).
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Flow Cyt | (1) |
1/50 - 1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 2.5 µg/ml. Detects a band of approximately 190 kDa (predicted molecular weight: 138 kDa). |
Flow Cyt
1/50 - 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. -
組織特異性
Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth. -
関連疾患
Hereditary diffuse gastric cancer
Glioma
Ovarian cancer
Lung cancer
Gastric cancer
Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172). -
細胞内局在
Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. - Information by UniProt
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参照データベース
- Entrez Gene: 403883 Dog
- Entrez Gene: 2064 Human
- Entrez Gene: 13866 Mouse
- Entrez Gene: 24337 Rat
- Omim: 164870 Human
- SwissProt: O18735 Dog
- SwissProt: P04626 Human
- SwissProt: P70424 Mouse
see all -
別名
- Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
- C erb B2/neu protein antibody
- CD340 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling ErbB2 / HER2 with ab16901 at 1/400 dilution. the tissue was fixed with formaldehyde; heat mediated antigen retrieval was performed using a acetate buffer pH6. An undiluted goat anti-mouse HRP conjugated seconday antibody was used.
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Anti-ErbB2 / HER2 antibody [3B5] (ab16901) at 1/250 dilution + Human Huh7 whole cell lysate at 20 µg
Secondary
Goat anti-mouse HRP conjugate at 1/5000 dilution
Predicted band size: 138 kDa
Observed band size: 138 kDa
Additional bands at: 48 kDa (possible non-specific binding)
Exposure time: 5 minutesBlocking: 5% milk for 1 hour at 25°C.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Mamma tumor Her2Neu tissue labeling ErbB 2 with ab16901.
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Anti-ErbB2 / HER2 antibody [3B5] (ab16901) at 1 µg/ml + Whole cell lysate HEK293
Predicted band size: 138 kDa -
Overlay histogram showing LoVo cells stained with ab16901 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16901, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in LoVo cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma labeling ErbB 2 with ab16901 at 1/100.
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Immunohistochemistry analysis of formalin fixed, paraffin embedded tissue section of human breast carcinoma labeling ErbB 2 with at16901 at dilution of 1/1000.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (63)
ab16901 は 63 報の論文で使用されています。
- Lim M et al. Landscape of Epidermal Growth Factor Receptor Heterodimers in Brain Metastases. Cancers (Basel) 14:N/A (2022). PubMed: 35158800
- Han D et al. The NF-κB/miR-488/ERBB2 axis modulates pancreatic cancer cell malignancy and tumor growth through cell cycle signaling. Cancer Biol Ther 23:294-309 (2022). PubMed: 35343383
- Sasamori H et al. Mutation Profiles of Ovarian Seromucinous Borderline Tumors in Japanese Patients. Curr Oncol 29:3658-3667 (2022). PubMed: 35621684
- Yücel D et al. Stimulation of Cardiomyocyte Proliferation Is Dependent on Species and Level of Maturation. Front Cell Dev Biol 10:806564 (2022). PubMed: 35663393
- Padmanabhan R et al. Quantification of the growth suppression of HER2+ breast cancer colonies under the effect of trastuzumab and PD-1/PD-L1 inhibitor. Front Oncol 12:977664 (2022). PubMed: 36568154