EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (ab216833)
製品の概要
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製品名
EMT Marker / Epithelial to Mesenchymal Transition Marker Panel -
種交差性
交差種: Human -
製品の概要
EMT Marker / Epithelial to Mesenchymal Marker antibody sampler panel ab216833 contains high performing antibodies against the key EMT markers: E-cadherin, N-cadherin, Snail + Slug, and Vimentin. It also contains an anti-rabbit secondary antibody.
EMT is a reversible process where epithelial cells undergo defined molecular changes to become motile mesenchymal cells. This EMT marker sampler panel is a cost-effective and convenient means of evaluating EMT.
The antibodies in this panel were selected for their exceptional performance in IHC in human tissues. These antibodies have also been tested in a number other applications and species. Please see the individual datasheets for additional information.
We do not recommend the use of SNAIL + SLUG antibody ab85936 for western blot (note: the other antibodies in this panel perform well in western blot). For SNAIL + SLUG in western blot, we recommend ab180714.
Panel contents:
E-cadherin rabbit monoclonal ab40772 - 10 µl
N-cadherin rabbit monoclonal ab76011 - 10 µl
Snail + Slug rabbit polyclonal ab85936 - 10 µg
Vimentin rabbit monoclonal ab92547 - 10 µl
anti-rabbit secondary antibody (HRP) ab205718 - 100 µg -
特記事項
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass CytometryTM. Please refer to the ‘Associated products’ section below.
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 packs ab40772 - Anti-E Cadherin antibody [EP700Y] 1 x 10µl ab76011 - Anti-N Cadherin antibody [EPR1791-4] 1 x 10µl ab85936 - Anti-SNAIL + SLUG antibody 1 x 10µg ab92547 - Anti-Vimentin antibody [EPR3776] 1 x 10µl ab205718 - Goat Anti-Rabbit IgG H&L (HRP) 1 x 100µg
関連製品
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Related Products
- Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab154207)
- Alexa Fluor® 488 Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab185013)
- Alexa Fluor® 488 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab185030)
- Anti-Vimentin antibody [EPR3776] - BSA and Azide free (ab193555)
- Alexa Fluor® 647 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab194719)
- Alexa Fluor® 647 Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab194982)
- Alexa Fluor® 647 Anti-N Cadherin antibody [EPR1791-4] (ab195186)
- Anti-E Cadherin antibody [EP700Y] - Low endotoxin, Azide free (ab201499)
- Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030)
- Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab202504)
- Alexa Fluor® 555 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab203428)
- Alexa Fluor® 555 Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab206878)
- Alexa Fluor® 594 Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab206880)
- PE Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab209446)
- Alexa Fluor® 405 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab210152)
- PE Anti-E Cadherin antibody [EP700Y] (ab224959)
- APC Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab224960)
画像
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Fluorescent immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab40772. Green-E-Cadherin red-PI
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IHC image of ab85936 staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85936, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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ab76011 staining N Cadherin in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA + 1% FBS for 2 hours at room temperature; antigen retrieval was by heat mediation in a citrate buffer pH6. Samples were incubated with primary antibody (1/500 in 1% BSA + 1% FBS) for 16 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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IHC image of unpurified ab92547 staining Vimentin in human breast adenocarcinoma formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92547, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab216833 は 1 報の論文で使用されています。
- Chen B et al. Exosomal miR-500a-5p derived from cancer-associated fibroblasts promotes breast cancer cell proliferation and metastasis through targeting USP28. Theranostics 11:3932-3947 (2021). PubMed: 33664871