Application
Western blot
Sample
Mouse Cell lysate - whole cell (endothelial cells, and liver)
Loading amount
10 µg
Specification
endothelial cells, and liver
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3
Other product details
Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Positive control
no bands!!!
Additional data
Abcam response
We have recommended some modifications to your protocol but have not received a reply from you. Of note, the problem might be solved by loading more protein samples e.g. 40-80ug/lane. Please let us know if our suggestions improved your results.
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 02 2007