Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Cryopreserved embryonic cerebellar neurons (QBMcel)
Permeabilization
No
Specification
Cryopreserved embryonic cerebellar neurons (QBMcel
Fixative
paraformaldehyde with picric acid
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10mM PBS with 0.3% Triton-X
Dilution
1/2000
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Donkey anti-rabbit AlexaFluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
QBM Cell Science cryopreserved rat cerebellar neurons cultured for 14 days on poly-D-lysine coated 96 well plates. Results were not as expected. Observed no immunofluorescence labeling.
Abcam response
Thank you for your valuable feedback. your data suggests that ab117521 may not be suitable to detect Endothelin A Receptor in rat cells under these experimental conditions. We hope this information is useful to our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Babben Tinner
Verified customer
投稿 Sep 29 2015