Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Other
Permeabilization
Yes - triton 1%
Specification
Brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C
Dilution
1/1000
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: ab150101
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Francoise Geffroy
Verified customer
投稿 Jan 11 2017