Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Liver)
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 20°C
Fixative
Acetone
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS
Dilution
1/50
Secondary antibody
Dilution
1/200
Name
Abcam antibody:Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed
Additional data
Additional Notes
0.1% Acetic Acid in Ethanol fixative was also successfully tested
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Fabio Marongiu
Verified customer
投稿 Oct 20 2015