Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain tissue)
Antigen retrieval step
None
Specification
Brain tissue
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10 · Temperature: 24°C
Fixative
Formaldehyde
Other product details
Incubation time
48 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS with 1%horse serum
Dilution
1/1000
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Biotinylated donkey anti-mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
This is an excellent antibody, however one has to be careful while doing Immunofluorescence. 4% PFA and Neutral buffered formalin work well however, post fixation has to be minimal 2-3 hours at room temperature.
Referenced in PMID: 23954779 and PMID: 24274907
PubMed ID
24274907: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Sumit Sarkar
Verified customer
投稿 Aug 14 2015