Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate pH=6
Sample
Rat Tissue sections (omentum)
Specification
omentum
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1%BSA in TBS
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Mouse IgG Antibody, HRP conjugate 12-349
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/500
Additional data
Additional Notes
This protocol was repeated with pepsin antigen retrieval , and also for skin wound and pancreas tissue sections, no staining was observed.
Abcam response
Thank you for your valuable feedback. This customer was sent a free of charge antibody replacement of their choice in compensation for this faulty antibody.
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Abcam user community
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投稿 Jan 09 2014