Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Heart)
Specification
Heart
Fixative
10% Neutral Buffered Formalin, 24h
Antigen retrieval step
Enzymatic
Permeabilization
No
Other product details
Concentration
2 µg/ml
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: DAKO with background reducing components
Secondary antibody
Name
Non-Abcam antibody was used: Southern Biotech anti-mouse IgG1
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Procedure carried out on a Ventana Discovery XT
Slides dewaxed offline through an alcohol series
Protease treatment was protease 1, 4 minutes
Primary incubation was carried out using the 'no-heat' setting.
Detection system was DABMap (streptavidin-HRP-DAB)
Slides dewaxed offline through an alcohol series
Protease treatment was protease 1, 4 minutes
Primary incubation was carried out using the 'no-heat' setting.
Detection system was DABMap (streptavidin-HRP-DAB)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 23 2012