Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Lung Artery)
Specification
Lung Artery
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Prot K
Permeabilization
No
Other product details
Dilution
1/25
Incubation time
1 hour(s) and 0 minute(s) · Diluent: BSA in TBS
Secondary antibody
Name
Non-Abcam antibody was used: RbaMs IgG then Envision Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
The antibody showed no staining in any elastic fibers in several rat tissues. The optimized protocol for human tissue also showed no staining in rat tissue. Heat retrievals were also tested with negative results. This antibody seems to not be reactive with rat elastin.
Abcam response
Thank you for your honest opinion, even if the results are negative. We encourage our customers to send us their results as this is valuable information.
However, the antibody has not been tested in the species, so further testing results with rats are very welcome.
However, the antibody has not been tested in the species, so further testing results with rats are very welcome.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jul 11 2012