Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate buffer (pH6)
Permeabilization
No
Specification
Lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in PBS
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Dako Envision + Dual Link System - HRP (K4061)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
The antibody was positive for elastic fibres in the lamina propria surrounding bronchioles and the elastic laminae of blood vessels in sheep lung. Also performed IHC-P on decalcified sheep bone and it also performed well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 11 2016