Anti-EXOSC5/CML28 抗体 (ab69699)
Key features and details
- Mouse polyclonal to EXOSC5/CML28
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-EXOSC5/CML28 antibody
EXOSC5/CML28 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to EXOSC5/CML28 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human EXOSC5/CML28. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (AAH07742.1)
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ポジティブ・コントロール
- human kidney tissue lysate, EXOSC5/CML28 transfected 293T cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69699の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 25 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 25 kDa). |
ターゲット情報
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機能
Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. -
組織特異性
Highly expressed in a variety of hematopoietic and epithelial tumor cell lines, but not in normal hematopoietic tissues or other normal tissue, with the exception of testis. -
配列類似性
Belongs to the RNase PH family. -
細胞内局在
Nucleus > nucleolus. Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 56915 Human
- Omim: 606492 Human
- SwissProt: Q9NQT4 Human
- Unigene: 283741 Human
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別名
- Chronic myelogenous leukemia tumor antigen 28 antibody
- CML28 antibody
- EXOS5_HUMAN antibody
see all
画像
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Anti-EXOSC5/CML28 antibody (ab69699) at 1/500 dilution + human kidney lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 25 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted? -
All lanes : Anti-EXOSC5/CML28 antibody (ab69699) at 1/500 dilution
Lane 1 : EXOSC5/CML28 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 25 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted? -
ab69699 at 10µg/ml staining EXOSC5/CML28 in human Hela cells by ICC/IF.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (5)
ab69699 は 5 報の論文で使用されています。
- Zinoviev A et al. Extraction of mRNA from Stalled Ribosomes by the Ski Complex. Mol Cell 77:1340-1349.e6 (2020). PubMed: 32006463
- Hildebrandt MR et al. Cytoplasmic aggregation of DDX1 in developing embryos: Early embryonic lethality associated with Ddx1 knockout. Dev Biol 455:420-433 (2019). PubMed: 31330130
- Rubio K et al. Inactivation of nuclear histone deacetylases by EP300 disrupts the MiCEE complex in idiopathic pulmonary fibrosis. Nat Commun 10:2229 (2019). PubMed: 31110176
- Cammas A et al. Destabilization of nucleophosmin mRNA by the HuR/KSRP complex is required for muscle fibre formation. Nat Commun 5:4190 (2014). PubMed: 24969639
- Basu U et al. The RNA Exosome Targets the AID Cytidine Deaminase to Both Strands of Transcribed Duplex DNA Substrates. Cell 144:353-63 (2011). WB ; Human, Mouse . PubMed: 21255825