Anti-EXOC7 抗体 (ab118792)
Key features and details
- Rabbit polyclonal to EXOC7
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-EXOC7 antibody
EXOC7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EXOC7 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to a region within amino acids 1-50 of Human EXOC7 (NP_001138769.1).
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ポジティブ・コントロール
- HeLa, 293T, Jurkat and NIH3T3 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab118792の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 83 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 83 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. In adipocytes, plays a crucial role in targeting SLC2A4 vesicle to the plasma membrane in response to insulin, perhaps directing the vesicle to the precise site of fusion. -
配列類似性
Belongs to the EXO70 family. -
ドメイン
The N-terminus is involved in SEC8 and ARHQ binding.
The C-terminus is required for translocation to the plasma membrane. -
細胞内局在
Cytoplasm > cytosol. Cell membrane. Translocates, as a preformed complex with SEC6 and SEC8, to the plasma membrane in response to insulin through the activation of ARHQ. - Information by UniProt
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参照データベース
- Entrez Gene: 505568 Cow
- Entrez Gene: 23265 Human
- Entrez Gene: 53413 Mouse
- Entrez Gene: 64632 Rat
- Omim: 608163 Human
- SwissProt: Q3ZC43 Cow
- SwissProt: Q9UPT5 Human
- SwissProt: O35250 Mouse
see all -
別名
- 2 5 3p antibody
- DKFZp686J04253 antibody
- EX070 antibody
see all
画像
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All lanes : Anti-EXOC7 antibody (ab118792) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 83 kDa
Exposure time: 3 minutes -
Detection of EXOC7 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118792 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab118792 は 1 報の論文で使用されています。
- Coulter ME et al. Regulation of human cerebral cortical development by EXOC7 and EXOC8, components of the exocyst complex, and roles in neural progenitor cell proliferation and survival. Genet Med 22:1040-1050 (2020). PubMed: 32103185