Anti-ETFA 抗体 [2B11AE8] (ab110316)
Key features and details
- Mouse monoclonal [2B11AE8] to ETFA
- Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
Anti-ETFA antibody [2B11AE8]
ETFA 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2B11AE8] to ETFA -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, Flow Cyt, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Cow, Human -
免疫原
Recombinant full length protein. This information is considered to be commercially sensitive.
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ポジティブ・コントロール
- ICC: HeLa cells. Flow Cyt: HL-60 cells. IHC-P: Human heart tissue. WB: HepG2 and HEK-293T whole cell lysates; Human liver mitochondria; Bovine heart mitochondria; Rat liver mitochondria; Mouse liver mitochondria.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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精製度
Proprietary Purification -
特記事項(精製)
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
2B11AE8 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110316の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.
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ICC/IF |
Use a concentration of 4 µg/ml.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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IHC-P |
Use a concentration of 5 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. |
ICC/IF
Use a concentration of 4 µg/ml. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
IHC-P
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). -
関連疾患
Glutaric aciduria 2A -
配列類似性
Belongs to the ETF alpha-subunit/FixB family. -
ドメイン
Domain I shares an identical polypeptide fold with the beta subunit ETFB though there is no sequence similarity. -
翻訳後修飾
The N-terminus is blocked. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 521892 Cow
- Entrez Gene: 2108 Human
- Entrez Gene: 110842 Mouse
- Entrez Gene: 300726 Rat
- Omim: 608053 Human
- SwissProt: Q2KJE4 Cow
- SwissProt: P13804 Human
- SwissProt: Q99LC5 Mouse
see all -
別名
- Alpha ETF antibody
- Alpha-ETF antibody
- Electron transfer flavoprotein alpha polypeptide antibody
see all
画像
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All lanes : Anti-ETFA antibody [2B11AE8] (ab110316) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : ETFA knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDaLanes 1-2: Merged signal (red and green). Green - ab110316 observed at 35 kDa. Red - loading control ab52901 observed at kDa.
ab110316 Anti-ETFA antibody [2B11AE8] was shown to specifically react with ETFA in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266513 (knockout cell lysate ab257943) was used. Wild-type and ETFA knockout samples were subjected to SDS-PAGE. ab110316 and Anti-beta Tubulin [EP1331Y] - Microtubule Marker (ab52901) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-ETFA antibody [2B11AE8] (ab110316) at 1 µg/ml
Lane 1 : Human heart tissue
Lane 2 : HepG2 whole cells
Lane 3 : Human liver mitochondria
Lane 4 : Bovine heart mitochondria
Lane 5 : Rat liver mitochondria
Lane 6 : Mouse liver mitochondria
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDa -
Immunocytochemistry image of stained HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the antibody (ab110316, 4 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix
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HL-60 cells were stained with 1 µg/mL ab110316 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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IHC image of ETFA staining in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110316, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
データシートおよび資料
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Datasheet download
参考文献 (8)
ab110316 は 8 報の論文で使用されています。
- Andonian BJ et al. Rheumatoid arthritis T cell and muscle oxidative metabolism associate with exercise-induced changes in cardiorespiratory fitness. Sci Rep 12:7450 (2022). PubMed: 35523821
- Fiorentino TV et al. Pioglitazone corrects dysregulation of skeletal muscle mitochondrial proteins involved in ATP synthesis in type 2 diabetes. Metabolism 114:154416 (2021). PubMed: 33137378
- Ehrlicher SE et al. Mitochondrial adaptations to exercise do not require Bcl2-mediated autophagy but occur with BNIP3/Parkin activation. FASEB J 34:4602-4618 (2020). PubMed: 32030805
- Fisher-Wellman KH et al. Respiratory Phenomics across Multiple Models of Protein Hyperacylation in Cardiac Mitochondria Reveals a Marginal Impact on Bioenergetics. Cell Rep 26:1557-1572.e8 (2019). PubMed: 30726738
- White PJ et al. The BCKDH Kinase and Phosphatase Integrate BCAA and Lipid Metabolism via Regulation of ATP-Citrate Lyase. Cell Metab 27:1281-1293.e7 (2018). PubMed: 29779826
- Stauch KL et al. Loss of Pink1 modulates synaptic mitochondrial bioenergetics in the rat striatum prior to motor symptoms: concomitant complex I respiratory defects and increased complex II-mediated respiration. Proteomics Clin Appl 10:1205-1217 (2016). PubMed: 27568932
- Davoudi M et al. Complex I function and supercomplex formation are preserved in liver mitochondria despite progressive complex III deficiency. PLoS One 9:e86767 (2014). WB ; Mouse . PubMed: 24466228
- Pougovkina O et al. Mitochondrial protein acetylation is driven by acetyl-CoA from fatty acid oxidation. Hum Mol Genet N/A:N/A (2014). Mouse . PubMed: 24516071