Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) preadsorbed
Additional data
Additional Notes
Asynchronous cells were left untreated, or treated with either PBDu (1uM 30min) or U0126 (10uM 1 h), paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. Following PDBu treatment, this antibody appears slightly brighter within the cytoplasm and nucleus, whereas it is quite dim within untreated cells. Following U0126 treatment, there does not appear to be much of a difference in localization and/or labeling intensity.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Jul 26 2017