Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
Yes - 70% methanol
Sample
Human Cell (HEK293)
Specification
HEK293
Gating Strategy
live cells gated
Preparation
Cell harvesting/tissue preparation method: Cell dissociation buffer
Sample buffer: Enzyme free
Sample buffer: Enzyme free
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS + 0.2% BSA + 0.1% Sodium azide
Dilution
1/40
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG PE
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution
1/100
Additional data
Additional Notes
Purple plot represents isotype control, green plot represents ERK1 in HEK293 cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 04 2014