Anti-ERG 抗体 (ab96697)
Key features and details
- Rabbit polyclonal to ERG
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-ERG antibody
ERG 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ERG -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human ERG aa 445-457.
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ポジティブ・コントロール
- WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates ICC/IF: Human pancreatic cancer cell line
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96697の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Predicted molecular weight: 55 kDa.
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ICC/IF |
1/50.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 55 kDa. |
ICC/IF
1/50. |
ターゲット情報
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機能
Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure. -
関連疾患
Defects in ERG are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving ERG is found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1.
Note=Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS. Translocation t(X;21)(q25-26;q22) with ELF4. -
配列類似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
細胞内局在
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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参照データベース
- Entrez Gene: 2078 Human
- Omim: 165080 Human
- SwissProt: P11308 Human
- Unigene: 473819 Human
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別名
- Avian erythroblastosis virus E-26 (v-ets) oncogene related antibody
- D030036I24Rik antibody
- Erg 3 antibody
see all
画像
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Anti-ERG antibody (ab96697) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 55 kDa
10% SDS PAGE -
Immunofluorescence analysis of ERG in paraformaldehyde fixed Human pancreatic cancer cell line, using ab96697 at a 1/50 dilution.
Lower image: merged with DNA probe. -
Immunofluorescence analysis of ERG in paraformaldehyde fixed Human pancreatic cancer cell line, using ab96697 at a 1/50 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab96697 は 1 報の論文で使用されています。
- Polson ES et al. Monoallelic expression of TMPRSS2/ERG in prostate cancer stem cells. Nat Commun 4:1623 (2013). Human . PubMed: 23535644