Anti-ENO1 抗体 [2G2AG11BF8] (ab112994)
Key features and details
- Mouse monoclonal [2G2AG11BF8] to ENO1
- Suitable for: IP, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
製品の概要
-
製品名
Anti-ENO1 antibody [2G2AG11BF8]
ENO1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2G2AG11BF8] to ENO1 -
由来種
Mouse -
アプリケーション
適用あり: IP, Flow Cyt, ICC/IFmore details -
種交差性
交差種: Human
非交差種: Non human primates -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
-
ポジティブ・コントロール
- HeLa and HL60 cells; HeLa, Human Liver and Heart homogenates.
-
特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99.98% HEPES buffered saline -
Concentration information loading...
-
精製度
Proprietary Purification -
特記事項(精製)
ab112994 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (>95%). -
ポリ/モノ
モノクローナル -
クローン名
2G2AG11BF8 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
-
Assay kits
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab112994の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IP |
Use at an assay dependent concentration.
|
|
Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC/IF |
Use a concentration of 5 µg/ml.
|
特記事項 |
---|
IP
Use at an assay dependent concentration. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
-
機能
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. -
組織特異性
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
パスウェイ
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
配列類似性
Belongs to the enolase family. -
発生段階
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
翻訳後修飾
ISGylated. -
細胞内局在
Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line. - Information by UniProt
-
参照データベース
- Entrez Gene: 2023 Human
- Omim: 172430 Human
- SwissProt: P06733 Human
- Unigene: 517145 Human
-
別名
- 2 phospho D glycerate hydro lyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Alpha enolase antibody
see all
画像
-
Immunprecipitation of ENO1 by ab112994.
Staining of ENO1 (47 kDa) from HeLa cell lysate (1), Human liver (2) and Heart (3) homogenates. The identity of the protein was confirmed by mass spectrometry. This gel was stained with colloidal Coomassie blue G. -
ab112994 at 1ug/ml staining ENO1 in MeOH-fixed HL60 cells by Flow Cytometry(blue). Isotype control antibody (red).
-
ab112994 at 5ug/ml staining ENO1 in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green). The target locates mainly to the cytoplasm.
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (1)
ab112994 は 1 報の論文で使用されています。
- Chen X et al. Interaction between granulin A and enolase 1 attenuates the migration and invasion of human hepatoma cells. Oncotarget 8:30305-30316 (2017). PubMed: 28415822